Publications 45




The DRY motif and the four corners of the cubic ternary complex model 

Rovati GE, Capra V, Shaw VS, Malik RU, Sivaramakrishnan S, Neubig R. Cellular Signaling. 2017 Mar 24;35:16-23. doi: 10.1016/j.cellsig.2017.03.020. 
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Publications 44




Priming GPCR signaling through the synergistic effect of two G proteins 

Gupte TM, Malik RU, Sommese RF, Ritt M, Sivaramakrishnan S. PNAS. 2017, Mar 21 [Epub date] doi:10.1073/pnas.1617232114
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Publications 43


The GCaMP-R Family of Genetically Encoded Ratiometric Calcium Indicators 

Cho JH, Swanson CJ, Chen J, Li A, Lippert LG, Boye SE, Rose K, Sivaramakrishnan S, Chuong CM, Chow RH. ACS Chem. Biol. 2017 Feb 14 [Epub date] doi: 10.1021/acschembio.6b00883
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Publications 42



The role of regulatory domains in maintaining auto-inhibition in the multi-domain kinase PKCα 

Sommese RF, Ritt M, Swanson CJ, Sivaramakrishnan S. J. Biol. Chem. 2017, Jan 3: doi:10.1074/jbc.M116.768457
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Publications 41



Calcium Stimulates Self-Assembly of Protein Kinase C α In Vitro

C. Swanson, R. Sommese, K. Petersen, M. Ritt, J. Karslake, D. Thomas, and S. Sivaramakrishnan. PLoS ONE, 2016, Oct 5 11(10): e0162331. doi: 10.1371/journal.pone.0162331
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Publications 40




G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay

A. Semack, R. U. Malik, and S. Sivaramakrishnan. J. Vis. Exp., 2016, Sep 10 (115), e54696, doi:10.3791/54696 (2016).
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Publications 38




Patterning protein complexes on DNA nanostructures using a GFP nanobody

R. F. Sommese, R. F. Hariadi, K. Kim, M. Liu, M.J. Tyska, and S. Sivaramakrishnan. Protein Science. 2016 Nov; 25(11):2089-2094. doi: 10.1002/pro.3020 
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Publications 37



Engineering Circular Gliding of Actin Filaments Along Myosin-Patterened DNA Nanotube Rings To Study Long-Term Actin-Myosin Behaviors

R. Hariadi, A. Appukutty, and S. Sivaramakrishnan. ACS Nano, 2016, 10(9), 8281-8288 doi:10.1021/acsnano.6b01294
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Publications 39


Substrate Affinity Differentially Influences Protein Kinase C Regulation and Inhibitor Potency

R. Sommese and S. Sivaramakrishnan. J. Biol. Chem. 2016 Oct; 291(42):21963-21970
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Structural elements in the Gαs and Gαq C-termini that mediate selective GPCR signaling.

A. Semack*, M. Sandhu*, R. U. Malik, N. Vaidehi and S. Sivaramakrishnan. J. Biol. Chem. 2016, Aug; 291(34): 17929-17940 doi: 10.1074/jbc.M116.735720
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Correlation between Activity and Domain Complementation in Adenylyl Cyclase Demonstrated with a Novel FRET Sensor

M. Ritt and S. Sivaramakrishnan. Molecular Pharmacology. April 2016, 89 (4) 407-412; doi: 10.1124/mol.115.101626 
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Dynamic coupling and allosteric networks in the alpha subunit of heterotrimeric G proteins.

N.W. Griggs, L. Skjaerven, J.R. Traynor, S. Sivaramakrishnan, B.J. Grant. J. Biol. Chem. 2016, Feb; 291(9): 4742-4753 
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Using Protein Dimers to Maximize the Protein Hybridization Efficiency with Multisite DNA Origami Scaffolds.

Verma, L. Mallik, R.F. Hariadi, S. Sivaramakrishnan, G. Skiniotis, A.P. Joglekar. PLoS One 2015, Sep 8;10(9):e0137125. 
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Publications 32


Mechanical coordination in motor ensembles revealed using engineered artificial myosin filaments.

R. F. Hariadi*, R. F. Sommese*, A. S. Adhikari, R. E. Taylor, S. Sutton, J. A. Spudich and S. Sivaramakrishnan Nature Nanotechnology 2015, Aug;10(8), :696-700. *Equal contribution.
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PubImages 31



Cellular chirality arising from the self-organization of the actin cytoskeleton.

Y.H. Tee, T. Shemesh, V. Thiagarajan, R.F. Hariadi, K.L. Anderson, C. Page, N. Volkmann, D. Hanein, S. Sivaramakrishnan, M.M. Kozlov, A.D. Bershadsky. Nature Cell Biology 2015, 17(4), 445-57. 
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Publications 30




The C2 domain and altered ATP-binding loop phosphorylation at Ser359 mediate the redox-dependent increase in Protein Kinase Cδ activity.

J. Gong, Y. Yao, P. Zhang, B. Udayasuryan, E.V. Komissarova, J. Chen, S. Sivaramakrishnan, J.E. Van Eyk, S.F. Steinberg. Mol Cell Biol. 2015 May;35(10):1727-40. doi: 10.1128/MCB.01436-14.
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Publications 29




Tuning myosin-driven sorting on cellular actin networks.

R.F. Hariadi, R. Sommese, S. Sivaramakrishnan eLife 2015;4:e05472 DOI: 
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Publications 28




A method for multiprotein assembly in cells reveals independent action of kinesins in complex.

S. R. Norris, V. Soppina, A. S. Dizaji, K. I. Schimert, D. Sept, D. Cai, S. Sivaramakrishnan, and K. J. Verhey Journal of Cell Biology 2014, 207(3), 393-406.  
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Publications 27



Optofluidic lasers with a single molecular layer of gain.

Q. Chen, M. Ritt, S. Sivaramakrishnan, Y.A. Sun and X. Fan Lab Chip, 2014,14, 4590-4595 DOI:10.1039/C4LC00872C
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publications 26



Natural Killer Cells Eradicate Galectin-1-Deficient Glioma in the Absence of Adaptive Immunity.

C.J. Baker, P. Chockley, V.N. Yadav, R. Doherty, M. Ritt, S. Sivaramakrishnan, M.G. Castro, and P.R. Lowenstein. Cancer Research 2014, 15;74(18), 5079-90.
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publications 25



Harnessing the unique structural properties of isolated α-helices.

C.J. Swanson and S. Sivaramakrishnan Journal of Biological Chemistry 2014, 12;289(37), 25460-7.
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publications 24



Conserved Modular Domains Team Up to Latch-Open Active PKCα.

C.J. Swanson, M. Ritt, W. Wang, M. Lang, A. Narayan, J. Tesmer, M. Westfall, S. Sivaramakrishnan Journal of Biological Chemistry 2014, 289(25), 17812-29.
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Publiications 23




Myosin lever arm directs collective motion on cellular actin network.

R.F. Hariadi, M. Cale, and S. Sivaramakrishnan. Proceedings of the National Academy of Sciences 2014, 111(11), 4091-6.
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Publications 22




Analyses of conformational states of the transporter associated with antigen processing (TAP) in a native cellular membrane environment.

J. Geng, S. Sivaramakrishnan and M. Raghavan Journal of Biological Chemistry 2013, 288, 37039-47.
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Publications 21




Agonist Activated PKCβII Translocation and Modulation of Cardiac Myocyte Contractile Function.

H. Hwang, D. Robinson, J. B. Rogers, T. K. Stevenson, S. E. Lang, S. Sadayappan, S. M. Day, S. Sivaramakrishnan S, M. V. Westfall.Scientific Reports 2013, 3:1971.
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Publications 20




Detection of G Protein-selective G Protein-coupled Receptor (GPCR) Conformations in Live Cells.

R.U. Malik , M. Ritt, B.T. Devree, R.R. Neubig, R.K. Sunahara, S. Sivaramakrishnan. Journal of Biological Chemistry 2013, 288(24), 17167-78.
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Publications 19



Highly sensitive fluorescent protein FRET detection using optofluidic lasers.

Q. Chen , X. Zhang , Y. Sun , M. Ritt , S. Sivaramakrishnan and X. Fan. Lab on a Chip 2013, 13(14), 2679-81.
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Publications 18



Visualizing and manipulating focal adhesion kinase regulation in live cells.

M. Ritt, J.L. Guan, S. Sivaramakrishnan.Journal of Biological Chemistry 2013, 288(13), 8875-86.
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Publications 17




Cell-intrinsic functional effects of the α-cardiac myosin Arg-403-Gln mutation in familial hypertrophic cardiomyopathy.

P. Chuan, S. Sivaramakrishnan, E.A. Ashley, J.A. Spudich. Biophysical Journal 2012, 102(12), 2782-2790.
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Publications 16




Dual-beam optical tweezers.

S. Sivaramakrishnan, J. Sung, A. Dunn, J.A. Spudich. Encyclopedia of Biophysics, Ed. Gordon Roberts, Springer.
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Publications 15





Systematic control of protein interaction using a modular ER/K α-helix linker.

S. Sivaramakrishnan, J. A. Spudich,Proceedings of the National Academy of Sciences, USA 2011, 108(51), 20467-72.
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Publications 14




Principles of unconventional myosin function and targeting.

M. Hartman, D. Finan, S. Sivaramakrishnan, J. A. Spudich,Ann. Rev. Cell Dev. Biol 2011, 27, 133-55.
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2010 and earlier

Publications 13




Helicity of short E-R/K peptides.

R. F. Sommese, S. Sivaramakrishnan, R. L. Baldwin, and J. A. Spudich, Protein Science2010, 19(10), 2001-5.
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Publications 12





Single-molecule dual-beam optical trap analysis of protein structure and function.

J. Sung, S. Sivaramakrishnan, A. Dunn, J.A. Spudich, Methods in Enzymology 2010, 475, 321-75.
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Publications 11





Myosin VI: an innovative motor that challenged the swinging lever arm hypothesis.

S. Sivaramakrishnan, J.A. Spudich, Nature Reviews: Molecular and Cellular Biology 2010, 11(2), 128-37
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Publications 10




Combining single molecule optical trapping and small angle X-ray scattering measurements to compute the persistence length of a protein ER/K alpha-helix.

S. Sivaramakrishnan, J. Sung, M. Ali, S. Doniach, H. Flyvbjerg, J. A. Spudich, Biophysical Journal 2009, 97(1-7), 2993-9
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Publications 9




Coupled myosin VI motors facilitate unidirectional movement on an F-actin network.

S. Sivaramakrishnan, J.A. Spudich,Journal of Cell Biology 2009, 187(1), 53-60 DOI: 10.1083/jcb.200906133
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Publications 8



Insights into human beta-cardiac myosin function from single molecule and single cell studies.

S. Sivaramakrishnan, E. Ashley, L. Leinwand, J.A. Spudich, J. J Cardiovasc Transl Res. 2009 Dec; 2(4): 426–440. doi:  10.1007/s12265-009-9129-2
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Publications 5




Shear stress induced reorganization of the keratin intermediate filament network requires PKC zeta.

S. Sivaramakrishnan, J.L. Schneider, A. Sitikov, R.D. Goldman, K.M. Ridge, Molecular Biology of the Cell, 2009, 20(11), 2755-65.. 
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Publications 7



Dynamic charge interactions create surprising rigidity in the ER/K alpha-helical protein motif.

S. Sivaramakrishnan, B. J. Spink, A. Y. L. Sim, S. Doniach, J. A. Spudich, Proceedings of the National Academy of Sciences, USA, 2008, 105(36), 13356-61. 
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Publications 6



Long single alpha-helical tail domains bridge the gap between structure and function of myosin VI.

B.J. Spink, S. Sivaramakrishnan, J. Lipfert, S. Doniach, J.A. Spudich, Nature: Structural and Molecular Biology, 2008, 15(6), 591-97. 
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Publications 4



Micromechanical properties of keratin intermediate filament networks.

S. Sivaramakrishnan, J.V. DeGuilio, L. Lorand, R.D. Goldman, K.M. Ridge, Proceedings of the National Academy of Sciences, USA, 2008, 105(3), 2755-65.
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Publications 3




Computational and Experimental Study of Turbulent Flow in a 0.4-Scale Water Model of a Continuous Steel Caster.

Q. Yuan, S. Sivaramakrishnan, S.P. Vanka, B.G. Thomas, Metallurgical and Materials Transactions B, 2004, 35(5), 967-982(16).
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Publications 2




Transient Fluid Flow in the Continuous Steel-slab Casting Mold.

B.G. Thomas, Q. Yuan, S. Sivaramakrishnan, S.P. Vanka. Journal of Metals - Electronic Edition, 2002, 54(1).
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Publications 1




Comparison of four methods to evaluate fluid velocities in a continuous slab casting mold.

B.G. Thomas, Q. Yuan, S. Sivaramakrishnan, T. Shi, S.P. Vanka and M.B. Assar, ISIJ Int., 2001, 41(10), 1262-1271.
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