Kim 2021 cover art


β2-adrenoceptor ligand efficacy is tuned by a two-stage interaction with the Gαs C-terminus

Keehun Kim, Shayla Paulekas, Fredrik Sadler, Tejas Gupte, Michael Ritt, Matthew Dysthe, Nagarajan Vaidehi, Sivaraj Sivaramakrishnan
PNAS March 2021; 118(11)

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Nisha Science advances


KIF13A motors are regulated by Rab22A to function as weak dimers inside the cell

Nishaben M. Patel, Meenakshi Sundaram Aravintha Siva, Ruchi Kumari, Dipeshwari J. Shewale, Ashim Rai, Michael Ritt, Prerna Sharma, Subba Rao Gangi Setty, Sivaraj Sivaramakrishnan, and Virupakshi Soppina
Sci Advances 2021; Feb 3;7(6):eabd2054.doi: 10.1126/sciadv.abd2054

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JBC Kinase model


Kinase inhibitors allosterically disrupt a regulatory interaction to enhance PKCα membrane translocation

Lisa G. Lippert*, Ning Ma*, Michael Ritt*, Abhinandan Jain, Nagarajan Vaidehi, Sivaraj Sivaramakrishnan
J Biol Chem 2021; Jan 25;doi: 10.1016/j.jbc.2021.100339

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Methods in Enzymology 2021


ER/K-link-Leveraging a native protein linker to probe dynamic cellular interactions.

Tejas Gupte, Michael Ritt, Sivaraj Sivaramakrishnan
Methods in Enzymology 2021;647:173-208.

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JBC Dab2 model


Dynamic multimerization of Dab2-Myosin VI complexes regulates cargo processivity while minimizing cortical actin reorganization

Ashim Rai*, Duha Vang*, Michael Ritt, Sivaraj Sivaramakrishnan
J Biol Chem 2020;Dec 28;jbc.RA120.012703. (Selected as Editor's Pick)
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Allosteric modulation of adenosine A1 and cannabinoid 1 receptor signaling by G‐peptides

Anja M. Touma, Rabia U. Malik, Tejas Gupte, Sivaraj Sivaramakrishnan
Pharm Res Persp 2020 Dec;8(6):e00673.

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Bock Cell 2020


Optical Mapping of cAMP Signaling at the Nanometer Scale

Andreas Bock, Paolo Annibale, Charlotte Konrad, Annette Hannawacker, Selma E. Anton, Isabella Maiellaro, Ulrike Zabel, Sivaraj Sivaramakrishnan, Martin Falcke, and Martin J. Lohse
Cell 2020 Aug 25; 10, 4836

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gupte et al nature comms 2019


Minute-scale persistence of a GPCR conformation state triggered by non-cognate G protein interactions primes signaling

Tejas Gupte, Michael Ritt, Matthew Dysthe, Rabia Malik, and Sivaraj Sivaramakrishnan
Nature Communications 2019 Oct 23; 10, 4836

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Biochemistry 2019


Stiffness of Cargo–Motor Linkage Tunes Myosin VI Motility and Response to Load

Rachit Shrivastava, Ashim Rai, Murti Salapaka, and Sivaraj Sivaramakrishnan
Biochemistry 2019 Sept 11; 58, 47, 4721–4725 (Featured cover article)

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PNAS 2019 fig 1


Conformational plasticity of the intracellular cavity of GPCR-G-protein complexes leads to G-protein promiscuity and selectivity.

Manbir Sandhu, Anja M. Touma, Matthew Dysthe, Fredrik Sadler, Sivaraj Sivaramakrishnan, and Nagarajan Vaidehi
PNAS 2019 May 28; epub ahead of print
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Publication 51


Bitopic Inhibition of ATP and Substrate Binding in Ser/Thr Kinases through a Conserved Allosteric Mechanism

Ning Ma, Lisa G. Lippert, Titu Devamani, Benjamin Levy, Sangbae Lee, Manbir Sandhu, Nagarajan Vaidehi, and Sivaraj Sivaramakrishnan
Biochemistry 2018 Oct 18; 57(45): 6387–90
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Molecular Motors




Engineering Synthetic Myosin Filaments Using DNA Nanotubes

Sommese R, Sivaramakrishnan S
Molecular Motors: Methods in Molecular Biology 2018 Jul 4; 1805: 93-101
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Publication 50





Engaging myosin VI tunes motility, morphology, and identity in endocytosis

Ritt M, Sivaramakrishnan S
Traffic. 2018 Jun 4; 19:710–722
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Publications 49


Conserved salt-bridge competition triggered by phosphorylation regulates the protein interactome

Skinner JJ, Wang S, Lee J, Ong C, Sommese R, Sivaramakrishnan S, Koelmel W, Hirschbeck M, Schindelin H, Kisker C, Lorenz K, Sosnick TR, Rosner MR.
Proc Natl Acad Sci U S A. 2017 Dec 19;114(51):13453-13458 
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Distinct structural mechanisms determine substrate affinity and kinase activity of Protein Kinase Cα

Lee S, Devamani T, Song HD, Sandhu M, Larsen A, Sommese R, Jain A, Vaidehi N, Sivaramakrishnan S.
J Biol Chem. 2017 Sep 29;292(39):16300-16309
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Publications 47


ER/K Linked GPCR-G protein fusions systematically modulate second messenger response in cells

Malik RU, Dysthe M, Ritt M, Sunahara RK, Sivaramakrishnan S.
Sci Rep. 2017 Aug 10;7(1):7749 
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Publications 46


Actin turnover maintains actin filament homeostasis during cytokinetic ring contraction

Chew TG, Huang J, Palani S, Sommese R, Kamnev A, Hatano T, Gu Y, Oliferenko S, Sivaramakrishnan S, Balasubramanian MK.
J Cell Biol. 2017 Sep 4;216(9):2657-2667
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Publications 45


The DRY motif and the four corners of the cubic ternary complex model 

Rovati GE, Capra V, Shaw VS, Malik RU, Sivaramakrishnan S, Neubig R. 
Cell Signal. 2017 Jul;35:16-23
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Publications 44



Priming GPCR signaling through the synergistic effect of two G proteins 

Gupte TM, Malik RU, Sommese RF, Ritt M, Sivaramakrishnan S. 
Proc Natl Acad Sci U S A. 2017 Apr 4;114(14):3756-3761
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Publications 43


The GCaMP-R Family of Genetically Encoded Ratiometric Calcium Indicators 

Cho JH, Swanson CJ, Chen J, Li A, Lippert LG, Boye SE, Rose K, Sivaramakrishnan S, Chuong CM, Chow RH. 
ACS Chem Biol. 2017 Apr 21;12(4):1066-1074
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Publications 42


The role of regulatory domains in maintaining auto-inhibition in the multi-domain kinase PKCα 

Sommese RF, Ritt M, Swanson CJ, Sivaramakrishnan S. 
J Biol Chem. 2017 Feb 17;292(7):2873-2880
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Publications 41


Calcium Stimulates Self-Assembly of Protein Kinase C α In Vitro

Swanson CJ, Sommese RF, Petersen KJ, Ritt M, Karslake J, Thomas DD, Sivaramakrishnan S.
PLoS ONE, 2016, Oct 5 11(10): e0162331
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Publications 40


G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay

Semack A, Malik RU, Sivaramakrishnan S.
J Vis Exp. 2016, Sep 10;(115)
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Publications 38



Patterning protein complexes on DNA nanostructures using a GFP nanobody

Sommese RF, Hariadi RF, Kim K, Liu M, Tyska MJ, Sivaramakrishnan S. 
Protein Sci. 2016 Nov;25(11):2089-2094. 
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Publications 37


Engineering Circular Gliding of Actin Filaments Along Myosin-Patterened DNA Nanotube Rings To Study Long-Term Actin-Myosin Behaviors

Hariadi RF, Appukutty AJ, Sivaramakrishnan S. 
ACS Nano. 2016 Sep 27;10(9):8281-8
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Publications 39


Substrate Affinity Differentially Influences Protein Kinase C Regulation and Inhibitor Potency

Sommese RF, Sivaramakrishnan S. 
J Biol Chem. 2016 Oct; 291(42):21963-21970
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Structural elements in the Gαs and Gαq C-termini that mediate selective GPCR signaling.

Semack A, Sandhu M, Malik RU, Vaidehi N, Sivaramakrishnan S. 
J Biol Chem. 2016 Aug 19;291(34):17929-40
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Correlation between Activity and Domain Complementation in Adenylyl Cyclase Demonstrated with a Novel FRET Sensor

Ritt M, Sivaramakrishnan S. 
Mol Pharmacol. 2016 Apr;89(4):407-12
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Dynamic coupling and allosteric networks in the alpha subunit of heterotrimeric G proteins.

Yao XQ, Malik RU, Griggs NW, Skjaerven L, Traynor JR, Sivaramakrishnan S, Grant BJ. 
J Biol Chem. 2016 Feb 26;291(9):4742-53
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Using Protein Dimers to Maximize the Protein Hybridization Efficiency with Multisite DNA Origami Scaffolds.

Verma V, Mallik L, Hariadi RF, Sivaramakrishnan S, Skiniotis G, Joglekar AP. 
PLoS One. 2015 Sep 8;10(9):e0137125. 
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Publications 32


Mechanical coordination in motor ensembles revealed using engineered artificial myosin filaments.

Hariadi RF, Sommese RF, Adhikari AS, Taylor RE, Sutton S, Spudich JA, Sivaramakrishnan S.
Nat Nanotechnol. 2015 Aug;10(8):696-700
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PubImages 31


Cellular chirality arising from the self-organization of the actin cytoskeleton.

Tee YH, Shemesh T, Thiagarajan V, Hariadi RF, Anderson KL, Page C, Volkmann N, Hanein D, Sivaramakrishnan S, Kozlov MM, Bershadsky AD
Nat Cell Biol. 2015 Apr;17(4):445-57
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Publications 30


The C2 domain and altered ATP-binding loop phosphorylation at Ser359 mediate the redox-dependent increase in Protein Kinase Cδ activity.

Gong J, Yao Y, Zhang P, Udayasuryan B, Komissarova EV, Chen J, Sivaramakrishnan S, Van Eyk JE, Steinberg SF
Mol Cell Biol. 2015 May;35(10):1727-40
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Publications 29


Tuning myosin-driven sorting on cellular actin networks.

Hariadi RF, Sommese RF, Sivaramakrishnan S. 
Elife. 2015 Mar 4;4 
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Publications 28


A method for multiprotein assembly in cells reveals independent action of kinesins in complex.

Norris SR, Soppina V, Dizaji AS, Schimert KI, Sept D, Cai D, Sivaramakrishnan S, Verhey KJ
J Cell Biol. 2014 Nov 10;207(3):393-406
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Publications 27


Optofluidic lasers with a single molecular layer of gain.

Chen Q, Ritt M, Sivaramakrishnan S, Sun Y, Fan X
Lab Chip. 2014 Dec 21;14(24):4590-5
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publications 26


Natural Killer Cells Eradicate Galectin-1-Deficient Glioma in the Absence of Adaptive Immunity.

Baker GJ, Chockley P, Yadav VN, Doherty R, Ritt M, Sivaramakrishnan S, Castro MG, Lowenstein PR 
Cancer Res. 2014 Sep 15;74(18):5079-90
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publications 25


Harnessing the unique structural properties of isolated α-helices.

Swanson CJ, Sivaramakrishnan S. 
J Biol Chem. 2014 Sep 12;289(37):25460-7
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publications 24


Conserved modular domains team up to atch-open Active protein kinase Cα.

Swanson CJ, Ritt M, Wang W, Lang MJ, Narayan A, Tesmer JJ, Westfall M, Sivaramakrishnan S.
J Biol Chem. 2014 Jun 20;289(25):17812-29.
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Publiications 23


Myosin lever arm directs collective motion on cellular actin network.

Hariadi RF, Cale M, Sivaramakrishnan S.
Proc Natl Acad Sci U S A 2014, 111(11), 4091-6.
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Publications 22


Analyses of conformational states of the transporter associated with antigen processing (TAP) in a native cellular membrane environment.

Geng J, Sivaramakrishnan S, Raghavan M.
J Biol Chem.  2013 Dec 27;288(52):37039-47
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Publications 21



Agonist Activated PKCβII Translocation and Modulation of Cardiac Myocyte Contractile Function.

Hwang H, Robinson D, Rogers JB, Stevenson TK, Lang SE, Sadayappan S, Day SM, Sivaramakrishnan S, Westfall MV.
Sci Rep. 2013;3:1971
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Publications 20


Detection of G Protein-selective G Protein-coupled Receptor (GPCR) Conformations in Live Cells.

Malik RU, Ritt M, DeVree BT, Neubig RR, Sunahara RK, Sivaramakrishnan S.
J Biol Chem. 2013 Jun 14;288(24):17167-78
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Publications 19


Highly sensitive fluorescent protein FRET detection using optofluidic lasers.

Chen Q, Zhang X, Sun Y, Ritt M, Sivaramakrishnan S, Fan X. 
Lab Chip.  2013 Jul 21;13(14):2679-81
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Publications 18


Visualizing and manipulating focal adhesion kinase regulation in live cells.

Ritt M, Guan JL, Sivaramakrishnan S.
J Biol Chem. 2013 Mar 29;288(13):8875-86
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Publications 17


Cell-intrinsic functional effects of the α-cardiac myosin Arg-403-Gln mutation in familial hypertrophic cardiomyopathy.

Chuan P, Sivaramakrishnan S, Ashley EA, Spudich JA.
Biophys J. 2012 Jun 20;102(12):2782-90.
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Publications 16


Dual-beam optical tweezers.

Sivaramakrishnan S, Sung J, Dunn A, Spudich JA.
Encyclopedia of Biophysics, Ed. Gordon Roberts, Springer.
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Publications 15



Systematic control of protein interaction using a modular ER/K α-helix linker.

Sivaramakrishnan S, Spudich JA.
Proc Natl Acad Sci U S A. 2011 Dec 20;108(51):20467-72.
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Publications 14


Principles of unconventional myosin function and targeting.

Hartman MA, Finan D, Sivaramakrishnan S, Spudich JA.
Annu Rev Cell Dev Biol. 2011;27:133-55
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2010 and earlier

Publications 13


Helicity of short E-R/K peptides.

Sommese RF, Sivaramakrishnan S, Baldwin RL, Spudich JA. 
Protein Sci. 2010 Oct;19(10):2001-5.
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Publications 12


Single-molecule dual-beam optical trap analysis of protein structure and function.

Sung J, Sivaramakrishnan S, Dunn A, Spudich JA. 
Methods Enzymol 2010;475:321-75.
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Publications 11


Myosin VI: an innovative motor that challenged the swinging lever arm hypothesis.

S. Sivaramakrishnan, J.A. Spudich.
Nat Rev Mol Cell Biol. 2010 Feb;11(2):128-37
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Publications 10


Combining single molecule optical trapping and small angle X-ray scattering measurements to compute the persistence length of a protein ER/K alpha-helix.

Sivaramakrishnan S, Sung J, Ali M, Doniach S, Flyvbjerg H, Spudich JA.
Biophys J. 2009 Dec 2;97(11):2993-9
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Publications 9


Coupled myosin VI motors facilitate unidirectional movement on an F-actin network.

Sivaramakrishnan S, Spudich JA.
J Cell Biol. 2009 Oct 5;187(1):53-60
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Publications 8


Insights into human beta-cardiac myosin function from single molecule and single cell studies.

Sivaramakrishnan S, Ashley E, Leinwand L, Spudich JA.
J Cardiovasc Transl Res. 2009 Dec;2(4):426-40
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Publications 5


Shear stress induced reorganization of the keratin intermediate filament network requires PKC zeta.

Sivaramakrishnan S, Schneider JL, Sitikov A, Goldman RD, Ridge KM. 
Mol Biol Cell, 2009 Jun;20(11):2755-65.
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Publications 7


Dynamic charge interactions create surprising rigidity in the ER/K alpha-helical protein motif.

Sivaramakrishnan S, Spink BJ, Sim AY, Doniach S, Spudich JA.
Proc Natl Acad Sci U S A. 2008 Sep 9;105(36):13356-61 
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Publications 6


Long single alpha-helical tail domains bridge the gap between structure and function of myosin VI.

Spink BJ, Sivaramakrishnan S, Lipfert J, Doniach S, Spudich JA. 
Nat Struct Mol Biol 2008 Jun;15(6):591-7 
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Publications 4



Micromechanical properties of keratin intermediate filament networks.

Sivaramakrishnan S, DeGuilio JV, Lorand L, Goldman RD, Ridge KM.
Proc Natl Acad Sci U S A. 2008 Jan 22;105(3):889-94
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Publications 3



Computational and Experimental Study of Turbulent Flow in a 0.4-Scale Water Model of a Continuous Steel Caster.

Yuan Q, Sivaramakrishnan S, Vanka SP, Thomas BG.
Metallurgical and Materials Transactions. 2004, 35(5), 967-982(16).
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Publications 2



Transient Fluid Flow in the Continuous Steel-slab Casting Mold.

Thomas BG, Yuan Q, Sivaramakrishnan S, Vanka SP. 
Journal of Metals - Electronic Edition, 2002, 54(1).
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Publications 1



Comparison of four methods to evaluate fluid velocities in a continuous slab casting mold.

B.G. Thomas, Q. Yuan, S. Sivaramakrishnan, T. Shi, S.P. Vanka and M.B. Assar, ISIJ Int., 2001, 41(10), 1262-1271.
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